Journal: Theranostics

Article Title: Yap1 modulates cardiomyocyte hypertrophy via impaired mitochondrial biogenesis in response to chronic mechanical stress overload

doi: 10.7150/thno.74563

Figure Lengend Snippet: YAP1 activation is responsible for mitochondrial perturbation and cardiomyocyte hypertrophy. (A) Normalized enrichment scores (NESs) of gene sets related to YAP signaling and mitochondria. At 1-, 2-, and 3-month post AAC surgery, ventricular tissues were subjected to RNA-seq and gene set enrichment analysis (GSEA). N = 3 for each group. (B) Immunofluorescence staining for activated YAP (aYAP) in cardiac tissue sections and quantification of aYAP positive cardiomyocytes. Scale bar, 50 µm. N = 6 hearts per group. (C) Immunoblotting analysis for aYAP in cardiac tissues. N = 3 hearts per group. (D) Immunostaining for YAP and ACTN2 on iPSC derived cardiomyocytes tissue bundles which are subjected to normal and exceeded stretch. (E) The quantitative result of the ratio of YAP+ cardiomyocytes on iPSC derived tissue bundles. N = 8, each dot presents the average results of 5 fields/slice. Bar, 50 µm. (F). Experimental design to determine the role of YAP1 in AAC-induced cardiac responses. (G) Echocardiographic measurement of ventricular wall thickness in AAC-treated Yap1 F/F mice. Mean ± SD. N = 4 hearts per group. (H) WGA-stained cardiac cryosections and quantification of cardiomyocyte transverse section area. In AAC and AAV treated hearts, GFP+ and GFP- cardiomyocytes were separately measured. Scale bar, 50 µm. N = 3 hearts per group. (I) Measurement of the oxygen consumption rates (OCRs) of isolated GFP positive cardiomyocytes after FACS sorting. OCRs were normalized by total cardiomyocyte number per group. N = 9 hearts per group. Mean ± SD. In statistical analysis, student's t-test or Mann-Whitney U-test was applied. **P < 0.01. ***P < 0.001.

Article Snippet: The WTC human induced pluripotent stem cell (WTC-iPSC), which was described in previous research , was maintained in mTeSR1 (STEMCELL Technologies, 85850) and passaged by versene solution (Gibco, 15040-066).

Techniques: Activation Assay, RNA Sequencing, Immunofluorescence, Staining, Western Blot, Immunostaining, Derivative Assay, Isolation, MANN-WHITNEY